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. 2019 May 13;14(5):e0216946. doi: 10.1371/journal.pone.0216946

Fig 5. Anionic charges on the surface of TP4 oligomers.

Fig 5

(A) Binding of TP4 peptides to cationic (DE52) and anionic (CM52) resins. TP4 peptides were dissolved in sarkosyl solutions and centrifuged. The soluble fractions were incubated with DE52 or CM52 resins. The TP4 peptides in 0x Sar were pulled down by CM52 resins and those in 1x Sar were pulled down by DE52 resins. (B) Anionic charges on the surface of TP4 oligomer were altered by TFE. Various concentrations of TFE were added to TP4 peptides which are soluble in 1x Sar, and incubated with DE52 or CM52 resins. 30% TFE enabled anionic TP4 oligomers to bind CM52 resins at 1x Sar, but became unable to bind DE52 resins.