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. 2019 May 13;14(5):e0216946. doi: 10.1371/journal.pone.0216946

Fig 9. Conformational change of TP4 particles by sarkosyl and trifluoroethanol.

Fig 9

(A) Disruption of FITC-TP4 particles by sarkosyl. 1x Sar was prepared by the addition of equal volumes of 1.5x Sar (20 μl) to FITC-TP4 particles-containing drop (4 μg in 20 μl 0.5x Sar) on cover glass. Images were taken a by CLSM780 in a time course as demonstrated in the right panel with three fields (fluorescence, bright field, and merged image). (B) Formation of FITC-TP4 vesicles in 0.5x Sar. FITC-TP4 peptides (3 μg) were dissolved in 20 μl 1x Sar and loaded on glass. Equal volumes of 0x Sar was added to the drop and images were taken and shown as mentioned above. (C, D) Fusion and disruption of FITC-TP4 particles by TFE. FITC-TP4 particles (4 μg in 20 μl 0.5x Sar) were prepared as mentioned in panel (A). The 0.5x Sar solution was drained off and replaced with 20% TFE and 30% TFE. The FITC-TP4 particles fused with each other in 20% TFE (C) and dissolved in 30% TFE (D). (E) Susceptibility of TP4 vesicles to 30% TFE. The preformed TP4 particles (8 μg/75 μl 0.5x Sar) were resuspended in 100 μl TFE solution for 1hr with gentle shaking. The TP4 peptides in the supernatants and pellets after centrifugation were analyzed by SDS-PAGE and Coomassie Blue staining.