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. 2019 May 13;14(5):e0216946. doi: 10.1371/journal.pone.0216946

Fig 10. Assembly of TP4 peptides on the membrane of glutaraldehyde-fixed E. coli.

Fig 10

The 0.2% glutaraldehyde-fixed E. coli cells were treated with 40 μg TP4 peptides, crosslinked by 0.1% glutaraldehyde, extracted by 4x Sar and analyzed by SDS-PAGE. The bound TP4 peptides from the 4x Sar extract exhibited multiple bands (1–4) on the gel, which are similar to those induced by 1x Sar, but those from the insoluble pellet did not. Numbers 1, 2, 3 and 4 on the gel represent monomers, dimers, trimers and tetramers, respectively. Phosphate, 10 mM sodium phosphate pH 7.4; Sup, supernatant; 2nd GA, 0.1% glutaraldehyde; OmpC, outer membrane protein C; OmpX, outer membrane protein X.