Table 3. Mean percentages of viable Cryptosporidium and Giardia for different UV doses using microscopy.
Trials | UV dose (mJ/cm2) | |||||||||
5.2 mJ/cm2 | 10.4 mJ/cm2 | 20.8 mJ/cm2 | 41.6 mJ/cm2 | 83.2 mJ/cm2 | ||||||
Cryptosporidium (%) |
Giardia (%) |
Cryptosporidium (%) |
Giardia (%) |
Cryptosporidium (%) |
Giardia (%) |
Cryptosporidium (%) |
Giardia (%) |
Cryptosporidium (%) |
Giardia (%) |
|
1 | 53 | 20 | 33 | 0 | 6 | 0 | 6 | 0 | 2 | 0 |
2 | 41 | 29 | 17 | 13 | 5 | 0 | 2 | 0 | 1 | 0 |
3 | 46 | 20 | 20 | 0 | 5 | 0 | 2 | 0 | 2 | 0 |
4 | 47 | 33 | 20 | 17 | 5 | 0 | 2 | 0 | 0 | 0 |
5 | 46 | 28 | 19 | 15 | 4 | 0 | 3 | 0 | 0 | 2 |
Mean±SD | ||||||||||
Distilled water | 47.0±8.5 | 24.5±6.4 | 25.0±11.3 | 6.5±9.2 | 5.5±0.7 | 0.0±0.0 | 4.0±2.8 | 0.0±0.0 | 1.5±0.7 | 0.0±0.0 |
Wastewater | 46.3±0.6 | 27.0±6.6 | 19.7±0.6 | 10.7±9.3 | 4.7±0.6 | 0.0±0.0 | 2.3±0.6 | 0.0±0.0 | 0.7±1.2 | 0.7±1.2 |
Syto-9+PI | 46.7±6.0 | 23.0±5.2 | 23.3±8.5 | 4.3±7.5 | 5.3±0.6 | 0.0±0.0 | 3.3±2.3 | 0.0±0.0 | 1.7±0.6 | 0.0±0.0 |
DAPI+PI | 46.5±0.7 | 30.5±3.5 | 19.5±0.7 | 16.0±1.4 | 4.5±0.7 | 0.0±0.0 | 2.5±0.7 | 0.0±0.0 | 0.0±0.0 | 1.0±1.4 |
Note: Trials 1 to 2: Distilled water. Trials 3 to 5: Wastewater. Trials 1 to 3 were performed using Syto-9+PI stains and Trials 4 to 5 were performed using DAPI+PI stains.