Table 5. Comparison between the percentages of viability of (oo)cysts when using microscopy and flow cytometer for UV irradiations.
| UV dose (mJ/cm2) |
5.2 | 10.4 | 20.8 | 41.6 | 83.2 | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Trials | Microscopy | Flow cytometer | Microscopy | Flow cytometer | Microscopy | Flow cytometer | Microscopy | Flow cytometer | Microscopy | Flow cytometer |
| 1 | 50 | 43 | 30 | 12 | 6 | 9 | 6 | 8 | 2 | 3 |
| 2 | 40 | 43 | 17 | 12 | 4 | 10 | 2 | 8 | 1 | 3 |
| 3 | 44 | 45 | 18 | 12 | 5 | 10 | 2 | 9 | 2 | 3 |
| 4 | 46 | 43 | 20 | 11 | 5 | 10 | 2 | 9 | 0 | 3 |
| 5 | 43 | 45 | 19 | 12 | 5 | 10 | 5 | 9 | 2 | 3 |
| Mean±SD | 45±4 | 44±1 | 21±5 | 12±1 | 5±0 | 10±0 | 3±2 | 8±0 | 1±1 | 3±0 |
Note: Trials 1 to 2: Distilled water. Trials 3 to 5: Wastewater. Trials 1 to 3 were performed using Syto-9+PI stains and Trials 4 to 5 were performed using DAPI+PI stains.