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. 2019 May 13;14(5):e0216040. doi: 10.1371/journal.pone.0216040

Table 6. Comparison between the percentages of viability of (oo)cysts when using microscopy and flow cytometer for chlorine exposures.

0.5 ppm
DURATION
/min
15 30 60 120
Trials Microscopy flow cytometer Microscopy flow cytometer Microscopy flow cytometer Microscopy flow cytometer
1 83 85 75 76 51 71 40 67
2 85 82 73 80 58 72 46 67
3 82 77 68 81 45 70 41 63
4 89 83 70 79 57 69 50 69
5 86 81 58 78 54 72 45 66
MEAN±SD 85±3 82±3 69±6 79±1 53±6 71±1 44±4 66±3
2 ppm
1 79 85 63 76 57 71 46 67
2 77 82 70 80 61 72 53 67
3 70 77 61 81 52 70 44 63
4 77 83 70 79 63 69 54 69
5 84 81 62 78 54 72 45 66
MEAN±SD 77±6 82±3 65±5 79±1 57±5 71±1 48±5 66±3
5 ppm
1 42 40 33 31 24 21 14 12
2 32 42 28 32 20 21 16 13
3 42 41 33 33 24 21 11 12
4 39 42 30 35 21 21 14 13
5 50 42 30 33 24 23 17 13
MEAN±SD 41±7 41±1 31±2 33±1 23±2 21±1 15±3 12±0

Note: Trials 1 to 2: Distilled water. Trials 3 to 5: Wastewater. Trials 1 to 3 were performed using Syto-9+PI stains and Trials 4 to 5 were performed using DAPI+PI stains.