Table 6. Comparison between the percentages of viability of (oo)cysts when using microscopy and flow cytometer for chlorine exposures.
0.5 ppm | ||||||||
DURATION /min |
15 | 30 | 60 | 120 | ||||
Trials | Microscopy | flow cytometer | Microscopy | flow cytometer | Microscopy | flow cytometer | Microscopy | flow cytometer |
1 | 83 | 85 | 75 | 76 | 51 | 71 | 40 | 67 |
2 | 85 | 82 | 73 | 80 | 58 | 72 | 46 | 67 |
3 | 82 | 77 | 68 | 81 | 45 | 70 | 41 | 63 |
4 | 89 | 83 | 70 | 79 | 57 | 69 | 50 | 69 |
5 | 86 | 81 | 58 | 78 | 54 | 72 | 45 | 66 |
MEAN±SD | 85±3 | 82±3 | 69±6 | 79±1 | 53±6 | 71±1 | 44±4 | 66±3 |
2 ppm | ||||||||
1 | 79 | 85 | 63 | 76 | 57 | 71 | 46 | 67 |
2 | 77 | 82 | 70 | 80 | 61 | 72 | 53 | 67 |
3 | 70 | 77 | 61 | 81 | 52 | 70 | 44 | 63 |
4 | 77 | 83 | 70 | 79 | 63 | 69 | 54 | 69 |
5 | 84 | 81 | 62 | 78 | 54 | 72 | 45 | 66 |
MEAN±SD | 77±6 | 82±3 | 65±5 | 79±1 | 57±5 | 71±1 | 48±5 | 66±3 |
5 ppm | ||||||||
1 | 42 | 40 | 33 | 31 | 24 | 21 | 14 | 12 |
2 | 32 | 42 | 28 | 32 | 20 | 21 | 16 | 13 |
3 | 42 | 41 | 33 | 33 | 24 | 21 | 11 | 12 |
4 | 39 | 42 | 30 | 35 | 21 | 21 | 14 | 13 |
5 | 50 | 42 | 30 | 33 | 24 | 23 | 17 | 13 |
MEAN±SD | 41±7 | 41±1 | 31±2 | 33±1 | 23±2 | 21±1 | 15±3 | 12±0 |
Note: Trials 1 to 2: Distilled water. Trials 3 to 5: Wastewater. Trials 1 to 3 were performed using Syto-9+PI stains and Trials 4 to 5 were performed using DAPI+PI stains.