Fig 5. AS1842856 is a potent activator of HIV-1 LTR in human T cell.

(A) Experimental design. PBT were stimulated for 3 days with anti-CD3/CD28 beads and then infected with a pseudotyped HIV-1 retrovirus encoding GFP. Three days after infection, AS1842856 (500nM) or vehicle only were added to the culture. GFP expression levels were measured by flow cytometry two days later. (B) Result obtained with one representative donor (left panel) and mean results +/- SE of GFP expression measured in the GFP-positive gated cell population of 4 different donors (right panel) are shown. (C) Chronically HIV-1 infected H9 cells were cultured three days with AS1842856 or vehicle only. At the end of culture, GAG expression level was analyzed by flow cytometry. Result obtained in one experiment (left panel) and mean results +/- SE of 3 independent experiments (right panel) are shown. (D) ImageStream analysis of nuclear localization of NFAT1 in PBT treated for 7 days with AS1842856 (500nM) or vehicle only, either at the steady state, or after a 30 min stimulation with the calcium ionophore ionomycin. Results obtained with one representative donor are shown in the left panel, with the red bars representing the mean +/- SE of similarity score of at least 3x10³ individual cells (black dots) in each experimental condition. Mean +/- SE of these similarity scores obtained with 3 different donors are shown in the right panel.