Table 3. Panel of 20 single-mutation variants selected for affinity maturation of the anti-muCCL20 monoclonal antibody.

Mutations 2, 10, 14 and 16 were selected from Discovery Studio; 1, 3, 4, 5, 7, 8, 11, 12, 13, 17, 18 and 19 from Schrödinger; 5, 6, 9 and 15 from Rosetta and the neutral control mutation is 20.

Variant	Chain	Parent	IMGT	Mutation	Variant	Chain	Parent	IMGT	Mutation
1	H	S	28	R	11	H	N	113	R
2	H	S	29	D	12	H	F	115	H
3	H	T	36	Q	13	H	T	116	R
4	H	N	37	T	14	L	S	32	D
5	H	G	59	Y	15	L	D	34	Q
6	H	G	64	T	16	L	G	35	Y
7	H	A	65	Y	17	L	K	36	R
8	H	I	108	R	18	L	Y	38	R
9	H	T	110	W	19	L	F	114	R
10	H	T	110	F	20	L	Q	106	Y