Table 2. Particle size and concentration of nanoparticles by Nanoparticle Tracking Analysis (NTA).
Formulation | PBCAnp | PBCAnp-polB |
---|---|---|
Particles/ mL | 2.00 (± 0.00) x 1012 | 1.60 (± 0.10) x 1012 |
Size (nm) | 169.0 ± 1.1 | 166.0 ± 3.7 |
D10 (nm) | 114.0 ± 1.3 | 112.0 ± 3.1 |
D50 (nm) | 147.0 ± 1.9 | 150.0 ± 5.1 |
D90 (nm) | 225.0 ± 5.2 | 204.0 ± 3.8 |
Nanoparticles diluted in ultrapure water on 1: 1000 proportion, triplicate measurements, day 1. Control poly butyl-cyanoacrylate nanoparticles (PBCAnp) and nanoparticles loaded with polymyxin B 5 mg/mL (PBCAnp-polB); particle size distribution D10, D50, and D90 correspond to 10%, 50%, and 90% of total particles under the reported particle size value, respectively.