Fig 2. Steinernema IJs release toxic proteins.

(A) Silver stained protein gel of whole ESPs collected from S. feltiae IJs activated for 0 (non-exposed), 6, 12, 18, 24, and 30 hours in insect homogenate. All time course activations were done with approximately 2.5 million IJs and the collected ESPs were concentrated to the same volume (300 μl) and the same volume (3 μl) was loaded to each lane. (B) Silver stained protein gel of whole ESPs (1 μg) from S. feltiae (green) and S. carpocapsae (blue) activated for 0, 6, 12, and 18 hours. (C) Survival curves of flies injected with 20 ng of whole ESPs from S. feltiae. (D) Survival curves of flies injected with 20 ng of whole ESPs from S. carpocapsae (S. carpocapsae survival curve was recapitulated from Lu. et al, 2017). Each survival curve includes 3 or more biological replicates totaling at least 180 flies.