MacEwen 2017

Methods	Random allocation Single‐blind Study duration: 3 months Dropout: 11% Location: Australia Recruitment: through posters and word‐of‐mouth
Participants	Population: full‐time desk‐based employees in the Charlottetown area. Intervention group: 16 participants Control group: 12 participants Demographics: Mean age in years: intervention 43.2 (SD 9.7), control 48.9 (SD 11.4) BMI: intervention group 36.5 (SD 9) kg/m², control group 34.6 (SD 7) kg/m²
Interventions	Duration: 3 months Intervention: sit‐stand desk Control: no sit‐stand desk
Outcomes	Outcome name, measurement time/tool (units of measurement) Changes in sitting/standing/stepping time (minutes/8‐hour workday) measured at 12 weeks. Transitions in positions measured by activPAL3 accelerometer‐inclinometer Weight (kg), waist circumference (cm), BMI, body fat %, estimated V02max (ml/min/kg), systolic and diastolic BP (mmHg), fasting blood lipids (Total cholesterol/HDL/LDL/Triglycerides) (mmol/L), glucose (mmol/L), HbA1c (%), aortic augmentation Index (%), subendocardial variability (%) at 12 weeks
Notes	The project was supported by StepsCount, Inc
Risk of bias
Bias	Authors' judgement	Support for judgement
Random sequence generation (selection bias)	Low risk	Participants were randomly assigned via coin flip to intervention and control group.
Allocation concealment (selection bias)	Unclear risk	Not reported
Blinding of participants and personnel (performance bias) All outcomes	Unclear risk	Not reported
Blinding of outcome assessment (detection bias) All outcomes	Unclear risk	Not reported
Incomplete outcome data (attrition bias) All outcomes	High risk	Four participants were excluded from analysis (14% attrition).
Selective reporting (reporting bias)	Low risk	All outcomes mentioned in the study protocol were reported.
Baseline comparability/ imbalance	High risk	Participants in the control group were older (48.9 years, SD 11.4) than the intervention group (43.2 years, SD 9.7) and the intervention group had higher BMI (36.5 kg/m2, SD 9) than the control group (34.6 kg/m2 SD 7).
Validity of outcome measure	Low risk	activPal accelerometer is a valid instrument for assessing physical activity and sedentary behaviour