Skip to main content
. 2019 Apr 23;8:e44594. doi: 10.7554/eLife.44594

Figure 1. Internalization of L. interrogans into cells by CAV1/ITGB1-PI3K/FAK-mediated MF-dependent endocytosis.

(A) Leptospires in the cells infected with L. interrogans strain Lai for the indicated times, examined by confocal microscopy (scale bars = 5 μm). The blue plaques indicate the nucleus. The red spots around the nucleus indicate the intracellular leptospires. (B) Statistical summary of red fluorescence intensity reflecting the leptospires in the infected cells for the indicated times. Statistical data from experiments such as shown in (A). Bars show the means ± SD of three independent experiments. The red fluorescence intensity values from the cells without infection (before infection) were set as 1.0. (C) Lep-vesicles in the cells infected with L. interrogans strain Lai for 4 hr, examined by transmission electron microscopy (scale bars = 0.5 μm). The arrows indicate the intracellular leptospires in the membrane-bound vesicles. (D) Decrease of leptospires in the different inhibitor- or siRNA-treated cells infected with L. interrogans strain Lai for 4 hr, examined by confocal microscopy (scale bars = 5 μm). The legends are the same as shown in (A). (E) Statistical summary of red fluorescence intensity reflecting the leptospires in the different inhibitor- or siRNA-treated cells infected with L. interrogans strain Lai for 4 hr. Statistical data from experiments such as shown in (D). The other legends are the same as shown in (B).

Figure 1—source data 1. Representative source data for Figure 1B.
elife-44594-fig1-data1.xls (23.5KB, xls)
DOI: 10.7554/eLife.44594.005

Figure 1.

Figure 1—figure supplement 1. Depletion of target proteins in the cells.

Figure 1—figure supplement 1.

(A) Depletion of CAV1, ITGB1, ITGB2 or ITGB3 in the siRNA-interfered cells, determined by Western Blot assay. (B) Depletion of Rab11, Sec15, Sec3, VAMP2 or SYN1 in the siRNA-interfered cells, determined by Western Blot assay.
Figure 1—figure supplement 2. No decrease of leptospiral uptake in target protein-depleted cells.

Figure 1—figure supplement 2.

The leptospires in the Rab11-, Sec15-, Sec3-, VAMP2- or SYN1-delepted cells infected with L. interrogans strain Lai for 4 hr, examined by confocal microscopy (scale bars = 5 μm). The blue plaques indicate the nucleus. The red spots around the nucleus indicate the intracellular leptospires.