Skip to main content
. 2019 Apr 23;8:e44594. doi: 10.7554/eLife.44594

Figure 3. Early and recycling endosomes formation of Lep-vesicles by recruitment of Rab5 or Rab11/TfR.

(A) Co-localization of Lep-vesicles with Rab5 in the cells infected with L. interrogans strain Lai for the indicated times, determined by confocal microscopy (scale bars = 5 μm). The blue plaques indicate the nucleus. The red or green spots indicate the Lep-vesicles or early endosome marker Rab5. The yellow spots indicate the Lep-vesicle-Rab5 co-localization. The Lep-vesicle-Rab5 co-localization in the EOMA, HK-2 and BJ cells during infection was shown in the Figure 3—figure supplement 1A . (B) Statistical summary of Lep-vesicle-Rab5 co-localization percentages for the indicated times. Statistical data from experiments such as shown in (A). Bars show the means ± SD of three independent experiments. Two hundred cells in each experiment were analyzed to calculate the percentages. (C) Statistical summary of yellow fluorescence intensity reflecting the Lep-vesicle-Rab5 co-localization for the indicated times. The legends are the same as shown in (B) but for detection of the yellow fluorescence intensity (FI). The yellow FI values from the uninfected cells (before infection) were set as 1.0. (D) Co-localization of Lep-vesicles with Rab11 and TfR in the cells infected with L. interrogans strain Lai for the indicated times, determined by confocal microscopy (scale bars = 5 μm). The red, blue or green spots indicate the Lep-vesicles, recycling endosome marker Rab11 or TfR. The white spots indicate the Lep-vesicle-Rab11/TfR co-localization. The Lep-vesicle-Rab11/TfR co-localization in the EOMA, HK-2 and BJ cells during infection was shown in the Figure 3—figure supplement 1B . (E) Statistical summary of Lep-vesicle-Rab11/TfR co-localization percentages for the indicated times. Statistical data from experiments such as shown in (D). The other legends are the same as shown in (B) but for determination of Lep-vesicle-Rab11/TfR co-localization percentages. (F) Statistical summary of white fluorescence intensity reflecting the Lep-vesicle-Rab11/TfR co-localization for the indicated times. Statistical data from experiments such as shown in (D). The other legends are the same as shown in (C) but for detection of white FI.

Figure 3.

Figure 3—figure supplement 1. Early and recycling endosomes formation of Lep-vesicles by recruitment of Rab5 or Rab11/TfR.

Figure 3—figure supplement 1.

(A) Co-localization of Lep-vesicles with Rab5 in the cells infected with L. interrogans strain Lai for the indicated times, determined by confocal microscopy (scale bars = 5 μm). The blue plaques indicate the nucleus. The red or green spots indicate the Lep-vesicles or early endosome marker Rab5. The yellow spots indicate the Lep-vesicle-Rab5 co-localization. (B) Co-localization of Lep-vesicles with Rab11 and TfR in the cells infected with L. interrogans strain Lai for the indicated times, determined by confocal microscopy (scale bars = 5 μm). The red, blue or green spots indicate the Lep-vesicles, recycling endosome marker Rab11 or TfR. The legends are the same as shown in (A) but for detection of Lep-vesicle-Rab11/TfR co-localization.