Figure 7. Viability of released L. interrogans and infected cells.
(A) Viability of L. interrogans strain Lai released from the infected cells for the indicated times, examined by confocal microscopy (scale bars = 10 μm). The green leptospires are living and the red leptospires are dead. Almost no dead leptospires could be found. Viability of the spirochete released from the infected EOMA, HK-2 and BJ cells was shown in the Figure 7—figure supplement 1A . (B) Percentages of living L. interrogans strain Lai released from the infected cells for the indicated times, examined by spectrofluorometry. Bars show the means ± SD of three independent experiments. (C) Growth ability of L. interrogans strain Lai released from the infected cells in EMJH medium for a 7-d incubation at 28°C for the indicated times, determined by dark field microscopic Petroff-Hausser enumeration. Bars show the means ± SD of three independent experiments. The leptospiral inoculated dose was 107. (D) Viability of the cells during infection with L. interrogans strain Lai for the indicated times, determined by MTT. Bars show the means ± SD of three independent experiments. (E) No apoptotic/necrotic cells during infection with L. interrogans strain Lai for the indicated times, determined by flow cytometry. The annexin-V+/PI- cells are early-apoptotic and the annexin-V+/PI+ cells are post-apoptotic/necrotic. Camptothecin, a cellular apoptotic inducer, is used as the positive control. Almost no apoptotic/necrotic infected cells were found. Viability of the EOMA, HK-2 and BJ cells during infection with the spirochete by flow cytometry was shown in the Figure 7—figure supplement 1B .
Figure 7—figure supplement 1. Viability of released leptospires and infected cells.
