Figure 3. BtTLP adds U₋₁ to tRNA^His when Thg1 is deleted.

Counts and percentages of reads aligned to the −1 position, by base, or to the +1 position of tRNA^His. This RNA exhibits a significant change in the composition of 5’-ends between the ScThg1 and BtTLP samples as evident from the statistical significance (adjusted p-value <0.001) and large effect size (% change in read count). Most reads aligning near the 5’-end of tRNA^His contain the −1 nucleotide (reads that start with +1 indicated in gray), with almost all of them beginning with base G₋₁ (blue) in the ScThg1-expressing strain and base U₋₁ (red) in the BtTLP-complemented sample.