Table 1:
Changes in distribution of reads corresponding to 5’-ends of selected ncRNA
| ncRNA | Straina | −3 | −2 | A −1 | U −1 | C −1 | G −1 | +1 |
|---|---|---|---|---|---|---|---|---|
| tH(GUG) | ScThg1 | <1% | <1% | 2% | 5% | <1% | 74% | 19% |
| BtTLP | <1% | <1% | <1% | 92% | <1% | <1% | 7% | |
| snR47 | ScThg1 | <1% | 5% | <1% | 49% | <1% | <1% | 46% |
| BtTLP | <1% | 4% | <1% | 59% | <1% | <1% | 36% | |
| snR51 b | ScThg1 | <1% | 5% | <1% | 43% | <1% | <1% | 51% |
| BtTLP | <1% | 4% | <1% | 50% | <1% | <1% | 45% |
a
thg1Δ strain expressing indicated Thg1/TLP gene.
b
Although the change in snR51 peak patterns between the two strains (p-value ~0.07) is not statistically significant to the <0.05 level that was observed for the other two ncRNA, it follows a similar pattern (gain in representation of U−1 in going from the ScThg1- to the BtTLP-complemented sample) that was also validated by primer extension data (see Figure 4).