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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Autoimmun. 2019 Mar 8;100:52–61. doi: 10.1016/j.jaut.2019.02.006

Fig. 4. AS101 downregulates the expression of Th1 or Th17 lineage-specific transcription factors and the phosphorylation of downstream signaling molecules.

Fig. 4.

R161H LN cells were stimulated for 72 h with 2 μg/ml IRBP161-180 (IRBP) under Th1 or Th17 polarizing conditions in the absence (PBS) or presence of AS101 (0.1, 0.3, or 1 μg/ml). (A) After Th1 or Th17 polarization, CD3+CD4+ population was assessed for T-bet or RORγt expression, respectively, by flow cytometry. Results represent one of 3 independent experiments. (B) Th1- or Th17-polarized CD3+CD4+ population was assessed for pSTAT4 or pSTAT3, respectively. Results represent one of 2 independent experiments. (C) Th1- or Th17-polarized CD3+CD4+ population was assessed for pAKT expressing cells. No cytokine (Cyt) condition was used as a negative control. Results represent one of 2 independent experiments.