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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: J Cell Biochem. 2018 Nov 11;120(5):7309–7322. doi: 10.1002/jcb.28005

Figure 6. Metabolic effects of BETi are reversible.

Figure 6.

(A) Kasumi-1 cells were treated with BETi for 24, 48 or 72 hr and the cells allowed to recover for 0, 1 or 3 days. Viable cell numbers were graphed over time. (B) Oxygen consumption rate (OCR) and extracellular acidification rates (ECAR) were measured after treatment of Kasumi-1 cells for 2 days and allowed to recover in the absence of JQ1 for 3 or 5 days. For OCR, stage I injected Oligomycin (5 mM), stage II injected FCCP (1 mM), and stage III injected Rotenone/Antimycin A (0.5 mM). For ECAR, stage I injected Glucose (10 mM), stage II injected Oligomycin (5 mM), and stage III injected 2-Deoxyglucose (50 mM) (C) Kasumi-1 cells treated with BETi for 3 days (D3) and then allowed to recover for 3, 5 or 7 days without BETi were tested for cell size using forward scatter in FACS. (D) Normalized optical redox ratio (NAD(P)H/FAD) was assessed using optical metabolic imaging. A minimum of 125 cells per sample in biological triplicates were measured for DMSO- and JQ1-treated Kasumi-1 cells at 24–72 hours before washout (left panel) or for the 24–72 hours after washout (recovery) of JQ1 (right panel). **, p<0.01; ****, p<0.0001; Mann-Whitney test.