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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Mol Med (Berl). 2019 Mar 25;97(5):691–709. doi: 10.1007/s00109-019-01763-3

Fig 5.

Fig 5

Testisin proteolytic activity reduces ANG2 and ANGPTL4 gene expression in ES-2-Luc cells. qPCR validation study of genes identified to be up- or down-regulated at least 2-fold by the TaqMan Angiogenesis Array: a. ANG1, ANG2, ANGPTL4, PECAM1/CD31, ANGPTL2, PDGFB, VEGFA, and TIE2. mRNA expression was normalized to GAPDH and expressed relative to ES-2-Luc-Ctl cells. All graphs show mean +/− SEM and are the average of at least 2 independent experiments from triplicate wells, *p<0.05; **p<0.01; ***p<0.001, unpaired t-test. b. Measurement by ELISA of ANG2 and ANGPTL4 protein levels in conditioned media collected at 24 and 48 hrs, respectively. Graphs show mean +/− SEM and values are the average of at least 2 independent experiments. *p<0.05; **p<0.01; ***p<0.001, unpaired t-test.