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. 2019 May 13;9:7292. doi: 10.1038/s41598-019-43739-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secondary chimeric mice: Proportions of donor and residual recipient cells. Irradiated mice were transplanted with DPP9^S729A or DPP9-WT adult bone marrow cells along with CD45.1⁺ Ptprc^a adult BM cells at a ratio of 40:60 (donor:competitor). (a) The WT-origin group displayed a bias towards competitor cells over donor cells, whereas the DPP9^S729A-origin group displayed no bias. All groups had a shortfall below 100% (dotted line), which represented outlier competitor cells and residual recipient cells that were gated out during analysis. Mean, SD and individual data. Significance; ****p < 0.0001. (b) Proportions of leukocyte subpopulations for each mouse. WT-origin n = 9, DPP9^S729A-origin n = 10, at 6 weeks post-transplantation.