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Levels of the Selected Critical Factors
| Critical factor | Low level | High level | |
|---|---|---|---|
| PLGA amount (mg) | X1 | 30 | 60 |
| Liraglutide amount (mg) | X2 | 0.5 | 5 |
| 2nd sonication time (min) | X3 | 0.5 | 2 |
| PVA (%) | X4 | 0.5 | 2 |
| Lyoprotectant type | X5 | Mannitol | Trehalose |
| Lyoprotectant (%) | X6 | 5 | 10 |
| W2/O ratio | X7 | 2 | 5 |
