Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 13;7:14. doi: 10.1038/s41413-019-0054-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Heterozygous Hif-2α KO mice show increased bone mass. a–e Analysis of femoral trabecular or calvarial bones from 4-month-old Hif-2α^+/− and WT mice. mRNA levels of Hif-2α in femoral bone from WT (Hif-2α^+/+) and Hif-2α^+/− mice (n = 4; a). Representative images of µCT reconstructions of femoral trabecular and cortical bones (b) and H&E and TRAP staining of trabecular bones (scale bar: 100 μm; c). Bone volume per tissue volume (BV/TV), trabecular bone thickness (Tb.Th), trabecular separation (Tb.Sp), and trabecular number (Tb.N) were analyzed based on the µCT measurements (n = 8; b). BV/TV, the number of osteoblastic cells per bone perimeter (N.Ob/B.Pm), the osteoblast surface normalized by bone surface (Ob.S/BS), the number of osteoclastic cells per bone perimeter (N.Oc/B.Pm), and the osteoclast surface normalized by bone surface (Oc.S/BS) were assessed by bone histomorphometric analyses of the metaphyseal regions of femurs (n = 8; c). ELISA-based measurement of the serum concentrations of OCN (n = 5) and CTX-1 (n = 7) (d). The bone forming rate (BFR) and mineral apposition rate (MAR) were analyzed from measurements obtained using calcein double labeling of femurs (n = 4; e). f Representative µCT images and measurements of bone volume of calvarial defect models generated in Hif-2α^+/− and WT mice and in C57BL/6 mice infected with Ad-Hif-2α or Ad-control (Ad-C) (n = 8). g Representative images of µCT reconstructions of femoral trabecular bones are shown, and quantitative µCT analysis was used to measure the BV/TV, Tb.Th, Tb.Sp, and Tb.N of the femoral bones from OVX- or sham-operated mice (n = 8). Values are presented as the mean ± SEM (*P < 0.05, **P < 0.01, and ***P < 0.005). The effects of OVX and genetic deletion of Hif-2α as well as their interaction in mice were analyzed by two-way ANOVA (g BV/TV: interaction = 0.048 7, OVX < 0.000 1, genetic deletion of Hif-2α = 0.024 5)