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. 2019 May 13;7:14. doi: 10.1038/s41413-019-0054-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Osteoclast-specific depletion of HIF-2α increases bone mass. a Osteoclast-specific depletion of HIF-2α in Hif-2α^fl/fl and Hif-2α^fl/fl;Ctsk-Cre mice was determined by immunohistochemistry with anti-HIF-2α antibody (n = 3; scale bar: 10 μm). b, c Analysis of femoral trabecular bones from 4-month-old Hif-2α^fl/fl and Hif-2α^fl/fl;Ctsk-Cre mice. Quantitative µCT analysis of trabecular bones (n = 8; b), H&E and TRAP staining and bone histomorphometric analysis (n = 8; c). Scale bar: 100 μm. d, e Quantitative µCT analysis (n = 8; d) and measurement of serum OCN and CTX-1 concentrations (n = 6; e) in OVX or sham-operated Hif-2α^fl/fl and Hif-2α^fl/fl;Ctsk-Cre mice. f Schematic diagram depicting HIF-2α regulation of bone remodeling. Values are presented as the mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.005; ‘NS’ not significant). Scale bar: 100 μm. The effects of OVX and osteoclast-specific depletion of Hif-2α (cKO) as well as their interaction in mice were analyzed by two-way ANOVA (d BV/TV: interaction = 0.001 8, OVX < 0.000 1, cKO < 0.000 1; e OCN: interaction = 0.743 6, OVX < 0.000 1, cKO = 0.557 7; e CTX-1: interaction = 0.045 0, OVX < 0.000 1, cKO = 0.002 5)