Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 13;9:7264. doi: 10.1038/s41598-019-43756-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

XICs of transitions monitored for alkylated endogenous and heavy hepcidin in hepatocytes cell media (HepG2) in the presence (A) and absence of BMP6 (10 ng/ml) (B).The black arrows indicated the location of SRM peak apex based on the retention time of heavy internal standard. Transition legend at bottom left hand of figure defines the blue and purple XIC traces for y18 and y17, respectively. (C) L/H hepcidin ratios for both HepG2 control and with BMP6 obtained from triplicate analysis with the standard error of mean. (D) Secretion of hepcidin in conditioned media. Secreted hepcidin in conditioned media was measured using ELISA. Hepcidin quantity was normalized to 1 × 10⁶ cells.