Fig. 4.

Inter- and intraday repeatability of the TPNG analysis by CE-ESI-MS. The released N-glycans were analysed after sialic acid derivatization and reducing end labelling with GirP. The relative abundances of the 20 most abundant glycoforms are displayed (for a complete overview, see Supplementary Fig. 7), showing a median RSD of 6.8% within technical replicates form the same day and a median RSD of 9.4% over 3 days. To enable automated peak integration, the electropherograms were aligned prior to data extraction³². The relative glycan profiles and their variation were similar before and after alignment (Supplementary Figs. 10 and 11). Blue square: N-acetylglucosamine, green circle: mannose, yellow circle: galactose, red triangle: fucose, right pointing pink diamond: α2,6-linked N-acetylneuraminic acid, left pointing pink diamond: α2,3-linked N-acetylneuraminic acid, GirP: Girard’s reagent P label. Error bars represent the standard deviation (intraday n = 3, interday n = 9, independent technical experiments). Source data are provided as a Source Data file