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. 2019 May 13;9:7306. doi: 10.1038/s41598-019-43617-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of TLR2 is a potent inducer of IL-6 and sIL-6R release. (A,B) PBMCs were incubated with different TLR agonists for 24 h as indicated. Supernatants were collected and IL-6 or sIL-6R levels were determined via ELISA. Data shown are the mean ± SEM from four independent experiments, which were analyzed by one-way ANOVA followed by Dunnett’s Multiple Comparison test. Statistical significance compared to the unstimulated cells is indicated with an asterisk.