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. 2019 May 7;10:804. doi: 10.3389/fmicb.2019.00804

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Copyright © 2019 Macedo-Raygoza, Valdez-Salas, Prado, Prieto, Yamaguchi, Kato, Canto-Canché, Carrillo-Beltrán, Di Mascio, White and Beltrán-García.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SEM analysis of E. cloacae (Ec) and K. pneumoniae (Kp) incubated by 18 h. The left and right panels show SEM of Ec and Kp cells, respectively. (A,B) Surface of E. cloacae cells with lateral flagella, pili. Red circle in (B) indicates the area of the next magnification. (C) The red arrow indicates formation of outer membrane vesicles (OMV) on the surface of Ec cells, which is not present in Kp all over the bacterial cell at 2 μm. In (D–F) K. pneumoniae have a larger cell of 0.3–1.0 μm wide X 0.6–6.0 μm lengths, lateral flagella and pili. The bar scales are shown at the lower right of each microphotograph.