FIGURE 5.

Fluorescent detection of the expression of mrfp from the P_dsrLL (A) or P_dsrLS (B) promoter in the indicated LAB. Bacterial cultures grown in MRSG, MRSS, MRSM and MRSF at 20, 30, and 37°C were analyzed at the middle of the exponential phase by fluorescent microscopy. The specific fluorescence is depicted, and it was calculated as the ratio of the detected fluorescence (5×) and the bacterial biomass estimated from the A_{600 nm} of the culture. The ANOVA statistical analysis of the results is depicted. A p ≤ 0.05 was considered significant. Mean pairwise comparisons were computed with a Tukey’s test (α = 0.05). Means with the same letter were not significantly different.