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. 2019 May 13;10:2129. doi: 10.1038/s41467-019-10081-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Tubg1^Y92C/+ mice show abnormal cortical layering during development. a E18.5 cortices stained against SATB2 (gray), CTIP2 (magenta) and DAPI (blue). Vertical lines depict how cortical layers were considered for quantification, scale bar 50 µm. b Histograms represent the thickness of the cortical layers and CP (upper panel) and the cell density of layer V (lower panel). Data are from at least five embryos per condition, unpaired t-test, *p < 0.05, **p < 0.01. Right image shows heterotopic SATB2⁺ cells. Scale bar 50 µm. c EdU staining in the cortex. Cortical plate was divided into five equal bins from the intermediate zone to the marginal zone. White arrows show EdU-positives cells accumulation in the IZ. CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular zone/subventricular zone. Scale bar 100 µm. d Quantification of EdU-positive cells in the specified regions, n = 3 embryos per group, Two-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, ***p < 0.001. e Representative image of SATB2 staining in cortices of EdU injected Tubg1^Y92C/+ mice. Scale bar 50 µm. f Locomotory paths (colored lines) of electroporated neurons during time-lapse imaging. Scale bar 50 µm. g Percentage migrating neurons, n = 3 WT and n = 4 Tubg1^Y92C/+; h average migration velocity, i average number of pauses, and j. total time spent in pause, during the 10 h of recording, n = 4 independent experiments per group. Unpaired t-test, **p < 0.01, ***p < 0.001 compared to wild type mice. k Percentage electroporated bipolar cells persisting bipolar or transitioning to multipolar morphology in 10 h recordings (n = 3 WT, n = 4 Tubg1^Y92C/+ independent experiments per group). l Percentage electroporated multipolar cells persisting multipolar or transitioning to bipolar morphology in 10 h recordings (n = 3 WT, n = 4 Tubg1^Y92C/+, independent experiments per group). m Percentage of arrested electroporated bipolar neurons with the centrosome ahead of nucleus, n = 3 WT, n = 4 Tubg1^Y92C/+ independent experiments per group. Unpaired t-test, ***p < 0.001 compared to wild type mice. All data are presented as mean ± s.e.m. Source data are provided in the Source Data file