Fig. 2.

Depletion of EXOSC10 impairs RPA and RAD51 recruitment to DSBs. a The panel shows RAD51 immunofluorescent staining of HeLa cells depleted of either EXOSC10 or DIS3 for 48 h. The cells were fixed 30 min after UV laser micro-irradiation. The bar plot in the lower part of the figure shows the percentage of γH2AX-positive stripes that were co-stained by RAD51 (n > 35 cells analysed in each condition, from two independent experiments). b RPA immunofluorescent staining was performed on cells depleted of either EXOSC10 or DIS3 and fixed 15 min after UV micro-irradiation. The bar plot shows the percentage of γH2AX-positive stripes that were co-stained by RPA (n > 60 cells analysed for each siRNA treatment, from at least two independent experiments). c CtIP immunofluorescent staining was performed on cells depleted of either EXOSC10 or DIS3 and fixed 10 min after UV micro-irradiation. The bar plot shows the percentage of γH2AX-positive stripes that were co-stained by CtIP (n > 100 cells analysed for each siRNA treatment, from two independent experiments). In all panels, the error bars represent s.e.m. Statistical testing was done using a Mann–Whitney’s test and significant p-values are shown in the figure. The scale bars represent 20 μm. Source data for Fig. 2a–c are provided as a Source Data file