Fig. 1.
Anti-tumor function of PKR and eIF2α-P in mouse NEU breast cancer. a NEU wild type (WT; n = 40), NEU PKR−/− (n = 21) or NEU eIF2αS/A (n = 12) mice were monitored up to 180 days for breast tumor formation by palpation. T50 represents the time by which 50% of the mouse population develops the first palpable mammary tumor. ***p < 0.001. b, c The total number of tumor-bearing mammary glands, as well as the rate of mammary tumor outgrowth, which is represented by the average tumor volume (mm3), were assessed at 4 weeks after palpation for each of the indicated genotypes (n > 12). Data represent mean ± SEM ***p < 0.001. d Protein extracts of NEU breast tumors from 3 different mice per group were subjected to immunoblotting for the indicated proteins. Graphs indicate the quantification of data from 6 biological replicates. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001