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. 2019 May 13;9:7299. doi: 10.1038/s41598-019-43759-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MMP-2, MMP-9 and TIMP-1 assayed activity on different gel rigidities. (a) Extracellular activity of MMP-2 and MMP-9 from HSC conditioned media assayed by gelatin zymography. Signal intensity of the bands presented relative to 4 kPa sample. Data obtained from 6 independent experiments. Results are expressed as mean ± s.e.m. *Represents t test, p < 0.05, **p < 0.01, ***p < 0.001. (b) TIMP-1 activity assayed by reverse gelatin zymography. Signal intensity of the bands presented relative to 4 kPa sample. Data obtained from 6 independent experiments. Results are expressed as mean ± s.e.m. ***Represents t test, p < 0.001.