Figure 3.

Differences between MSD of mobile tracks can be explained by dwell times between experimental conditions and G-protein binding. (A) MSDs of mobile trajectories decreased after 10 min DAMGO treatment (10 µM, p = 0.031). No drug (n = 5855 trajectories), 1 min DAMGO (10 µM, n = 697 trajectories), 10 min DAMGO (10 µM, n = 3958 trajectories). (B) Example of a mobile track separated into free (blue) and confined (green) parts based on recurrence analysis (see materials and methods). A threshold value of 11 visits inside the circle was used to define confined portions (red dotted line). The right panel shows a time series of the number of visits inside the circle for the example track shown, and free and confined portions are colored as in the example track. When the trajectory is segmented into (C) confined and (D) free portions, the MSDs are no longer different between experimental conditions (confined p = 0.150, free p > 0.99). (E) Confined dwell times decrease after acute DAMGO treatment and increase with longer DAMGO treatment. Application of 100 ng PTX increases confined dwell times. **p < 0.01, Kruskall-Wallis one-way ANOVA with multiple comparisons using Bonferroni correction, error bars represent SEM for individual tracks.