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. 2019 May 13;9:7297. doi: 10.1038/s41598-019-43657-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colocalization with CCPs increases across all tracks after DAMGO (10 min) application. (A) When comparing all tracks, colocalization with CCPs increases after 10 min of DAMGO treatment (10 µM). No drug n = 9 cells, 670 trajectories; 10 min DAMGO n = 8 cells, 446 trajectories. (B) Distribution of distances to CCPs is shifted leftward after 10 min of DAMGO treatment (C) Combined data from (B) showing an overall decrease in the distance to CCPs for MORs treated with DAMGO for 10 min. ***p < 0.0001, Kruskall-Wallis one-way ANOVA with multiple comparisons using Bonferroni correction, error bars represent SEM for individual tracks.