Fig. 7.

GDV1-dependent increase in gametocyte-associated genes in Pfgdv1.gfp.dd.T1 parasites. a Schematic of the experiment indicating the Shld1 treatment time. The RNA collection times are indicated by black arrows (38 h Schizonts and 24 h later during the subsequent ring stage) and the grey arrow designates the time the Giemsa-stained smear was made to assess gametocyte production. b–e The fold change in the expression of each gene (2^−ΔΔC_T) in Pfgdv1.gfp.dd.T1 (b, c) or wild type NF54 (d, e) parasites at schizont- (b, d) or ring- (c, e) stages in the presence and absence of Shld1 is shown. The Shld1 minus schizont stage value for each gene is used as a reference and the mean ± s.e.m. of triplicate or quadruplicate flasks from three independent experiments is plotted. A two-way ANOVA with Sidak’s multiple comparisons test followed by a Bonferroni post-hoc test to correct for the analysis of 13 genes was used to determine significance and probability is indicated, p ≤ 0.05 (*), p ≤ 0.01 (**). The individual 2^−ΔΔC_T for each gene are shown in Supplementary Fig. 5