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. 2019 May 13;9:7260. doi: 10.1038/s41598-019-43677-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IQM-PC330 promotes ATF6-dependent transcriptional activation. (a) Concentration-dependent blockade of DREAM-ATF6 interaction by IQM-PC330. Coimmunoprecipitation of Flag-ATF6 (bZIP fragment aa 306–369) and Myc-DREAM (aa 71–256) proteins after overexpression in HEK293-T cells. Immunoprecipitated band (arrowhead) corresponds to Flag-ATF6. Coimmunoprecipitation was performed in the absence or presence of DMSO or IQM-PC330 (30 and 100 nM). Below, optical density ratio of the ATF6 band relative to the input. (b) Real-time qPCR analysis of Xbp1 and BiP mRNAs in STHdhQ^7/7 cells alone or after stimulation (14 h) with thapsigargin (Th, 50 nM) and in each condition pre-treated with vehicle (DMSO) or IQM-PC330 (PC330, 100 nM; for 1 h). Values are normalized relative to HPRT mRNA levels. The experiment was repeated three times in triplicate. **p = 0.0015 and ***p = 0.0002, (Ordinary Two-way ANOVA, Holm-Sidak multiple comparison test).