Fig. 2.
Acid extrusion is not severely disturbed in explanted spiral ligament fibrocytes of P12 Slc4a10 knockout mice. a pHi recordings from WT and KO spiral ligament fibrocytes (mean from recordings of 24 KO and 15 WT fibrocytes from ≥ 5 mice per genotype). The intracellular acid load was induced by bath application of 20 mM propionate (horizontal bar). Upward deflections indicate an increase in the pHi. b Columns represent the maximal peak acidosis (delta pHi) induced by propionate and amplitudes of the alkaline overshoot during propionate washout, respectively (mean ± standard error of means). No difference in the maximal peak acidosis in response to propionate (ΔpH WT, 0.137 ± 0.0342; ΔpH KO, 0.131 ± 0.0528; T38, t = 0.406, p = 0.69, two-sided Student’s t test) and the alkaline overshoot after propionate removal (ΔpH WT, 0.149 ± 0.0372; KO, 0.145 ± 0.0652; T38, t = 0.541, p = 0.85, two-sided Student’s t test) suggests that Slc4a10 does not play a major role for acid extrusion at the time window analyzed