Fig. 3.

Early onset auditory impairment and reduction of the endocochlear potential in Slc4a10 knockout mice. a Hearing thresholds were determined by auditory brain stem recordings in response to click stimuli. Compared with wild-type littermates (dotted gray line), hearing thresholds of Slc4a10 knockout mice (dotted black line) are significantly decreased at 2, 6, 26, and 52 weeks of age. Repeated-measures two-way ANOVA with Bonferroni post-test, *** p < 0.0005. Boxes depict 25th, median, and 75th percentiles and whiskers the 5th/95th percentile range. b In knockout mice, the endocochlear potential is decreased to 45.2 ± 7.9 mV compared with 102.7 ± 6.9 mV in wild-type mice (KO n = 6, WT n = 5) at 2 weeks of age. It remains significantly below wild-type levels at 3 weeks (KO 54.3 ± 8.7 mV vs 110.6 ± 3.3 mV; KO n = 10, WT n = 5) and 6 weeks of age (KO 68.0 ± 7.1 mV vs 112.2 ± 9.9 mV; KO n = 4, WT n = 3). Repeated-measures two-way ANOVA with Bonferroni post-test, ***p < 0.0005. Vertical bars indicate standard deviations