Fig. 7.

Expression of Slc4a7 is lost in spiral ligament fibrocytes upon disruption of Slc4a10. a Immunoblot analysis shows lack of Slc4a7 expression in protein lysates of spiral ligaments of Slc4a10 knockout mice and decreased Slc4a7 protein abundance in heterozygous (HET) Slc4a10 knockout mice at the age of 3 and 6 weeks of age (samples were pooled from n = 3 mice for each genotype and age). b Slc4a7 protein abundance in the brain, kidney, or pancreas protein lysates is preserved in 56-week-old Slc4a10 knockout mice but absent in spiral ligament lysates (n = 3 per genotype). c, d The Slc4a7 labeling (magenta) of fibrocytes is lost in Slc4a10 knockout mice (n = 2 mice). e, f The Slc4a10 labeling of spiral ligament fibrocytes (green) is preserved in Slc4a7 knockout mice (n = 2 mice). Nuclei are stained with DAPI (blue). Scale bars, c–f 100 μm