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. 2019 May 7;10:928. doi: 10.3389/fimmu.2019.00928

Figure 2.

Figure 2

Microbiota downregulate intestinal epithelial expression of C-type lectin Clec2e. (A) Relative Clec2e expression levels in IECs harvested from small intestine of GF and CNV mice, represented as reads per kilobase per million mapped reads (RPKM). (B,C) Real-time quantitative PCR analysis of Clec2e in IECs from GF and CNV mice isolated from (B) small intestine (SI) or (C) large intestine (LI). (D) Intestinal organoids derived from GF and CNV mice. (E) Clec2e mRNA expression in GF and CNV organoids. (F) Clec2e mRNA expression in GF intestinal organoids in the absence or presence of LPS. Data represent two independent experiments with 3–4 mice per group. Results are mean ± SEM. *p < 0.05, **p < 0.01.