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. 2019 May 7;10:928. doi: 10.3389/fimmu.2019.00928

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Copyright © 2019 Woo, Eshleman, Rice, Whitt, Vallance and Alenghat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Clec2e enables increased adherence of bacterial pathogens. Clec2e overexpressed in HEK293T cells using a FLAG-tagged Clec2e expression vector. (A) Western blot analysis of Clec2e-FLAG expression in vehicle or Clec2e-FLAG transfected HEK293T cell lysates. (B,C) In vitro bacterial adhesion assay of infected HEK293T cells, expressed as adherent CFUs 6 h post infection. (D) Immunofluorescent staining of HEK293T cells infected with GFP-expressing C. rodentium (green). Mammalian cell membrane and nuclei are stained with Phalloidin (red) and DAPI (blue), respectively. Scale bars, 25 μm. (E) MFI of GFP-C. rodentium bound to vehicle-treated or Clec2e-transfected HEK293T cells. Data represent 2 independent experiments with n = 3 per group. Results are mean ± SEM. ^*p < 0.05, ^***p < 0.001.