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Microbiota induce histone deacetylation and HDAC3 enrichment at Clec2e gene. (A) ChIP-seq for H3K9Ac in primary IECs isolated from the small intestine of GF and CNV mice. (B) ChIP-qPCR for H3K9Ac in Clec2e from IECs. (C) ChIP-qPCR for HDAC3 in Clec2e as percent of input, relative to a negative control gene (Insulin 1). (D) HDAC3 ChIP-qPCR in Clec2e from GF and CNV IECs. Data represent two independent experiments with 3-4 mice per group. Results are mean ± SEM. *p < 0.05.
