Figure 5.

HDAC3 regulates epithelial Clec2e expression and pathogen adherence. (A) ChIP-seq for H3K9Ac in primary IECs isolated from the large intestine of HDAC3^FF and HDAC3^ΔIEC mice. (B) ChIP-qPCR for H3K9Ac in Clec2e in IECs from the small intestine. (C) Clec2e expression in IECs from large and small intestine of HDAC3^FF and HDAC3^ΔIEC mice. (D) C. rodentium CFUs in stool at day 6 post infection. (E) Fluorescence microscopy of colon from HDAC3^FF and HDAC3^ΔIEC mice infected with GFP-C. rodentium. (Green: GFP-C. rodentium, Red: Phalloidin, Blue: DAPI). Scale bars, 25 μm. (F) MFI of GFP-C. rodentium infected intestinal organoids derived from HDAC3^FF and HDAC3^ΔIEC mice. Data are representative of at least 2 independent experiments with 3–4 mice per group. Results are mean ± SEM. ^*p < 0.05.