Fig. 5.

EDP increase the uptake of Dil-oxLDL in human macrophages through the ERC and its NEU1 subunit. a Human adherent macrophages were incubated with kE (50 µg/mL), DANA (400 µM), DANA + kE, V14 + kE (molar ratio 2:1) or V14 peptide alone for 1 h at 37 °C prior to the addition of 10 µM Dil-oxLDL (4 h, 37 °C). Cells were then washed, fixed and visualized by a fluorescent microscope. A representative field is shown. The inset corresponds to a zoom area of the kE condition and white arrows show the presence of DiI-oxLDL particles inside the cells. b The mean gray value per field for each condition was calculated and normalized to the respective control (w/o, DANA, V14). Results are expressed as mean ± SEM of six independent experiments (ten different fields/experiment). Statistical analysis was performed by Student’s t test (***p < 0.001; ns non-significant)