Table 1.
Protein identification by LC–MS/MS
| UnitProt accession number | Protein name | % coverage | Number of unique peptide | Average molecular mass (kDa) | Band no. |
|---|---|---|---|---|---|
| Membrane-bound proteins | |||||
| Q96CX2|KCD12_HUMAN | BTB/POZ domain-containing protein KCTD12 | 16 | 4 | 35.7 | 7 |
| Q9UGN4|CLM8_HUMAN | CMRF35-like molecule 8 | 9 | 2 | 33.2 | 16, 17 |
| O75131|CPNE3_HUMAN | Copine-3 | 6 | 2 | 60.1 | 13 |
| Q99961|SH3G1_HUMAN | Endophilin-A2 | 12 | 4 | 41.5 | 11 |
| P54753|EPHB3_HUMAN | Ephrin type-B receptor 3 | 2 | 2 | 110.3 | 5 |
| Q3B8N2|LEG9B_HUMAN | Galectin-9B | 10 | 3 | 39.7 | 1, 21, 23 |
| Q6DKI2|LEG9C_HUMAN | Galectin-9C | 10 | 3 | 39.6 | 1, 21, 23 |
| P48307|TFPI2_HUMAN | Tissue factor pathway inhibitor 2 | 10 | 2 | 26.9 | 9, 10 |
| Transmembrane proteins | |||||
| P20273|CD22_HUMAN | B-cell receptor CD22 | 11 | 8 | 95.3 | 22 |
| P21730|C5AR1_HUMAN | C5a anaphylatoxin chemotactic receptor 1 | 8 | 2 | 39.3 | 12, 22 |
| P08962|CD63_HUMAN | CD63 antigen | 7 | 2 | 25.6 | 15 |
| P60033|CD81_HUMAN | CD81 antigen | 12 | 2 | 25.8 | 2 |
| P16671|CD36_HUMAN | Platelet glycoprotein 4 (CD36) | 14 | 5 | 53.1 | 20, 21 |
| Q15438|CYH1_HUMAN | Cytohesin-1 | 19 | 2 | 46.4 | 14 |
| Q8WXG9|GPR98_HUMAN | G-protein-coupled receptor 98 | 1 | 3 | 693.1 | 19 |
| P18462|1A25_HUMAN | HLA class I histocompatibility antigen, A-25 alpha chain | 8 | 2 | 41.2 | 15 |
| P30450|1A26_HUMAN | HLA class I histocompatibility antigen, A-26 alpha chain | 8 | 2 | 41.1 | 15 |
| P30512|1A29_HUMAN | HLA class I histocompatibility antigen, A-29 alpha chain | 8 | 2 | 40.9 | 15 |
| P16189|1A31_HUMA | HLA class I histocompatibility antigen, A-31 alpha chain | 8 | 2 | 41.0 | 15 |
| P10314|1A32_HUMAN | HLA class I histocompatibility antigen, A-32 alpha chain | 17 | 4 | 41.0 | 15, 16 |
| P16190|1A33_HUMAN | HLA class I histocompatibility antigen, A-33 alpha chain | 8 | 2 | 40.9 | 15 |
| P30453|1A34_HUMAN | HLA class I histocompatibility antigen, A-34 alpha chain | 8 | 2 | 41.1 | 15 |
| P30456|1A43_HUMAN | HLA class I histocompatibility antigen, A-43 alpha chain | 8 | 2 | 41.0 | 15 |
| P30457|1A66_HUMAN | HLA class I histocompatibility antigen, A-66 alpha chain | 8 | 2 | 41.1 | 15 |
| P01891|1A68_HUMAN | HLA class I histocompatibility antigen, A-68 alpha chain | 8 | 2 | 40.9 | 15 |
| P30459|1A74_HUMAN | HLA class I histocompatibility antigen, A-74 alpha chain | 17 | 4 | 40.9 | 15, 16 |
| P04222|1C03_HUMAN | HLA class I histocompatibility antigen, Cw-3 alpha chain | 27 | 2 | 40.9 | 13 |
| P30505|1C08_HUMAN | HLA class I histocompatibility antigen, Cw-8 alpha chain | 44 | 2 | 40.8 | 13 |
| P23467|PTPRB_HUMAN | Receptor-type tyrosine-protein phosphatase beta | 1 | 2 | 224.3 | 12 |
| Q12913|PTPRJ_HUMAN | Receptor-type tyrosine-protein phosphatase eta | 3 | 3 | 145.9 | 20 |
| Signaling | |||||
| P31947|1433S_HUMAN | 14–3–3 protein sigma | 5 | 2 | 27.8 | 13 |
| Q99996|AKAP9_HUMAN | A-kinase anchor protein 9 | 0 | 2 | 453.7 | 13 |
| Q12802|AKP13_HUMAN | A-kinase anchor protein 13 | 0 | 3 | 307.6 | 3, 4, 7, 10, 13, 15 |
| P62158|CALM_HUMAN | Calmodulin | 41 | 8 | 16.8 | 1 |
| P01112|RASH_HUMAN | GTPase HRas | 12 | 2 | 21.3 | 10 |
| P01111|RASN_HUMAN | GTPase NRas | 12 | 2 | 21.2 | 10 |
| Q9UMX6|GUC1B_HUMAN | Guanylyl cyclase-activating protein 2 | 32 | 7 | 23.4 | 1, 3, 5, 9 |
| Q16539|MK14_HUMAN | Mitogen-activated protein kinase 14 | 19 | 5 | 41.3 | 13 |
| Q9Y314|NOSIP_HUMAN | Nitric oxide synthase-interacting protein | 30 | 7 | 33.2 | 11 |
| P49593|PPM1F_HUMAN | Protein phosphatase 1F | 44 | 20 | 49.8 | 16, 17 |
| Q13188|STK3_HUMAN | Serine/threonine-protein kinase 3 | 10 | 4 | 56.3 | 18 |
| O00506|STK25_HUMAN | Serine/threonine-protein kinase 25 | 12 | 2 | 48.1 | 17 |
| Q9P289|STK26_HUMAN | Serine/threonine-protein kinase 26 | 19 | 4 | 46.5 | 15 |
| Q86Y07|VRK2_HUMAN | Serine/threonine-protein kinase VRK2 | 7 | 3 | 58.1 | 16 |
| Q9UKE5|TNIK_HUMAN | TRAF2 and NCK-interacting protein kinase | 8 | 3 | 154.9 | 17, 19 |
| P07948|LYN_HUMAN | Tyrosine-protein kinase Lyn | 5 | 2 | 58.6 | 16 |
| Q12866|MERTK_HUMAN | Tyrosine-protein kinase Mer | 8 | 7 | 110.2 | 23 |
| Q9UGJ0|AAKG2_HUMAN | 5′-AMP-activated protein kinase subunit gamma-2 | 3 | 2 | 63.1 | 13 |
| Q96JB6|LOXL4_HUMAN | Lysyl oxidase homolog 4 | 7 | 4 | 84.5 | 19 |
| P01033|TIMP1_HUMAN | Metalloproteinase inhibitor 1 | 11 | 2 | 23.2 | 5 |
| Cytoskeleton | |||||
| O15143|ARC1B_HUMAN | Actin-related protein 2/3 complex subunit 1B | 20 | 6 | 41.0 | 12 |
| P59998|ARPC4_HUMAN | Actin-related protein 2/3 complex subunit 4 | 21 | 4 | 19.7 | 1 |
| Q562R1|ACTBL_HUMAN | Beta-actin-like protein 2 | 39 | 2 | 42.0 | 12, 13 |
| Q86UX7|URP2_HUMAN | Fermitin family homolog 3 | 6 | 2 | 76.0 | 18, 19, 22 |
| Q9UGP4|LIMD1_HUMAN | LIM domain-containing protein 1 | 11 | 5 | 72.2 | 19 |
| P19105|ML12A_HUMAN | Myosin regulatory light chain 12A | 30 | 5 | 19.8 | 112 |
| O14950|ML12B_HUMAN | Myosin regulatory light chain 12B | 30 | 5 | 19.8 | 1 |
| P24844|MYL9_HUMAN | Myosin regulatory light polypeptide 9 | 17 | 3 | 19.8 | 1 |
| P07737|PROF1_HUMAN | Profilin-1 | 19 | 2 | 15.1 | 13 |
| Q13464|ROCK1_HUMAN | Rho-associated protein kinase 1 | 3 | 4 | 158.2 | 23 |
| Q71U36|TBA1A_HUMAN | Tubulin alpha-1A chain | 9 | 3 | 50.1 | 1, 2, 10, 11 |
| Q6PEY2|TBA3E_HUMAN | Tubulin alpha-3E chain | 28 | 3 | 49.9 | 13 |
| Q9BVA1|TBB2B_HUMAN | Tubulin beta-2B chain | 5 | 2 | 49.9 | 11 |
| P04350|TBB4A_HUMAN | Tubulin beta-4A chain | 32 | 4 | 49.6 | 11, 12 |
| Vesicle trafficking | |||||
| Q92928|RAB1C_HUMAN | Putative Ras-related protein Rab-1C | 37 | 5 | 22.1 | 1, 4 |
| Q92546|RGP1_HUMAN | RAB6A-GEF complex partner protein 2 | 12 | 4 | 42.5 | 13, 14 |
| Q5HYI8|RABL3_HUMAN | Rab-like protein 3 | 22 | 6 | 26.4 | 7 |
| Q9H0U4|RAB1B_HUMAN | Ras-related protein Rab-1B | 37 | 5 | 22.2 | 1, 4 |
| Q14964|RB39A_HUMAN | Ras-related protein Rab-39A | 26 | 5 | 25.0 | 1 |
| O43752|STX6_HUMAN | Syntaxin-6 | 9 | 2 | 29.2 | 10 |
Trypsin-digested protein bands from two independent experiments were analyzed by nano-LC-ESI MS/MS and proteins identified with Mascot software in Swiss-Prot and trEMBL databases