Skip to main content
. 2019 May 7;13:198. doi: 10.3389/fncel.2019.00198

FIGURE 5.

FIGURE 5

Activation of phospholipid synthesis in membranes from a human glioblastoma multiforme (GM). Homogenates were obtained from a human GM and from adjacent non pathological tissue (NP) excised from the same patient. TH: total homogenate; MF: microsomal membrane fraction; MF+KCl: 1M KCl stripped microsomal membrane fraction; MF+KCl+c-Fos: stripped MF plus recombinant c-Fos. Results are the mean ± SD of three experiments performed in triplicate; non-pathological TH values were taken as 100%. Inset: Western Blot for c-Fos content in the samples assayed; Tubulin staining is used as a loading control. Note the high content of c-Fos in the GM sample as compared to the almost undetectable levels in the NP sample. Adapted from Silvestre et al., 2010 (this is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited).