Table 3.
List of proteomic findings.
| Accession | Pep. count/ uniq. Pep. | Anova (p) | Description | Localization | Fold of regu-lation | Log2 | Function |
|---|---|---|---|---|---|---|---|
| P15502 | 1/1 | 0.02 | Elastin (ELN) | ECM | 112.37 | 6.81 | Extracellular matrix structural constituent |
| Q9GZT8 | 2/1 | 0.00 | NIF3-like protein 1 (NIF3L1) | Nucleus | 70.76 | 6.14 | Negatively regulating the expression of genes involved in neuronal differentiation |
| P09486 | 2/2 | 0.00 | SPARC (SPARC) | ECM | 32.77 | 5.03 | Regulates cell growth through interactions with the extracellular matrix and cytokines |
| P20472 | 7/5 | 0.00 | Parvalbumin alpha (PVALB) | Nucleus | 15.34 | 3.94 | Involved in muscle-relaxation after contraction |
| P35052 | 1/1 | 0.00 | Glypican-1 (GPC1) | ECM | 9.51 | 3.25 | Binds collagen and participates in Schwann cell myelination |
| Q8N414 | 1/1 | 0.04 | PiggyBac transposable element-derived protein 5 (PGBD5) | Nucleus | 7.63 | 2.93 | Targets regulatory elements and tumor suppressor genes and promotes cell transformation |
| Q16363 | 18/17 | 0.02 | Laminin subunit alpha-4 (LAMA4) | ECM | 5.42 | 2.44 | Mediates attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components |
| P02452 | 34/31 | 0.04 | Collagen alpha-1(I) chain (COL1A1) | ECM | 5.06 | 2.34 | Extracellular matrix structural constituent |
| P08123 | 15/15 | 0.04 | Collagen alpha-2(I) chain (COL1A2) | ECM | 4.93 | 2.30 | Extracellular matrix structural constituent |
| O00468 | 5/5 | 0.02 | Agrin (AGRN) | Synaptic cleft | 4.77 | 2.25 | Formation and the maintenance of the NMJ and postsynaptic differentiation |
| Q8WVJ2 | 2/2 | 0.00 | NudC domain-containing protein 2 (NUDCD2) | Cyto-skeleton | 4.66 | 2.22 | Regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone |
| Q04917 | 10/5 | 0.04 | 14-3-3 protein eta (YWHAH) | Cytosol | 4.17 | 2.06 | Promotes myosin II turnover in the cell cortex and modulates cortical tension, cell shape, and cytokinesis (doi: 10.1074/jbc.M117.819391) |
| P62888 | 4/4 | 0.05 | 60S ribosomal protein L30 (RPL30) | Cytosol | 4.14 | 2.05 | Protein synthesis |
| P29400 | 2/1 | 0.05 | Collagen alpha-5(IV) chain (COL4A5) | ECM | 3.90 | 1.96 | Extracellular matrix structural constituent |
| P12829 | 14/11 | 0.02 | Myosin light chain 4 (MYL4) | Cyto-skeleton | 3.75 | 1.91 | Regulatory light chain of myosin |
| Q14314 | 2/2 | 0.02 | Fibroleukin (FGL2) | ECM | 3.38 | 1.75 | Playa a role in physiologic lymphocyte functions |
| P02766 | 9/8 | 0.03 | Transthyretin (TTR) | ECM | 2.98 | 1.58 | Protects against misfolding and the formation of amyloid fibrils |
| Q14247 | 2/2 | 0.01 | Src substrate cortactin (SRC8_HUMAN) | Plasma membrane | 2.92 | 1.55 | Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones |
| P08697 | 5/5 | 0.05 | Alpha-2-antiplasmin (SERPINF2) | ECM | 2.91 | 1.54 | Serine protease inhibitor |
| P23229 | 2/2 | 0.04 | Integrin alpha-6 (ITGA6) | Plasma membrane | 2.90 | 1.54 | Integrin alpha-6/beta-1 is a receptor for laminin |
| P50454 | 14/14 | 0.03 | Serpin H1 (SERPINH1) | Endoplas-mic Reticulum | 2.80 | 1.48 | Chaperone in the biosynthetic pathway of collagen |
| Q9BZQ8 | 5/5 | 0.02 | Protein Niban (FAM129A) | Endoplas-mic Reticulum | 2.75 | 1.46 | Involved in the Endoplasmic Reticulum stress response |
| Q15417 | 5/5 | 0.05 | Calponin-3 (CNN3) | Cyto-skeleton | 2.66 | 1.41 | Thin filament-associated protein that is implicated in the regulation and modulation of muscle contraction |
| P04003 | 12/10 | 0.04 | C4b-binding protein alpha chain (C4BPA) | ECM | 2.62 | 1.39 | Controls the classical pathway of complement activation |
| P50895 | 10/10 | 0.01 | Basal cell adhesion molecule (BCAM) | Plasma membrane | 2.62 | 1.39 | Laminin alpha-5 receptor |
| P40261 | 8/8 | 0.04 | Nicotinamide N-methyltransferase (NNMT) | Cytoplasm | 2.54 | 1.35 | Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions |
| Q15651 | 3/1 | 0.02 | High mobility group nucleosome-binding domain-containing protein 3 (HMGN3) | Nucleus | 2.45 | 1.29 | Regulates chromatin-dependent processes such as transcription, DNA-replication and -repair |
| Q8N3D4 | 3/3 | 0.04 | EH domain-binding protein 1-like protein 1 (EHBP1L1) | Endosome | 2.39 | 1.26 | Rab effector protein playing a role in vesicle trafficking |
| Q9UEY8 | 3/3 | 0.03 | Gamma-adducin (ADD3) | Plasma membrane | 2.35 | 1.23 | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network and plays a role in actin filament capping |
| Q8NC56 | 1/1 | 0.05 | LEM domain-containing protein 2 (LEMD2) | Nucleus | 2.34 | 1.22 | Involved in nuclear structure organization |
| P02760 | 10/10 | 0.04 | Protein AMBP (AMBP) | ECM | 2.24 | 1.16 | Elastase inhibitor |
| Q96AQ6 | 19/18 | 0.01 | Pre-B-cell leukemia transcription factor-interacting protein 1 (PBXIP1) | Nucleus | 2.20 | 1.14 | Tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling |
| Q14BN4 | 5/5 | 0.03 | Sarcolemmal membrane-associated protein (SLMAP) | Plasma membrane | 2.10 | 1.07 | Plays a role during myoblast fusion |
| O43488 | 4/4 | 0.05 | Aflatoxin B1 aldehyde reductase member 2 (AKR7A2) | Golgi apparatus | 2.09 | 1.07 | Plays an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB) |
| P14649 | 25/22 | 0.03 | Myosin light chain 6B (MYL6B) | Cytoskeleton | 2.08 | 1.05 | Regulatory light chain of myosin |
| P09960 | 11/10 | 0.03 | Leukotriene A-4 hydrolase (LTA4H) | Cytoplasm | 0.53 | −0.92 | Biosynthesis of proinflammatory mediator leukotriene B4 |
| Q16775 | 6/6 | 0.04 | Hydroxyacylglutathione hydrolase, mitochondrial (HAGH) | Mito-chondria | 0.53 | −0.92 | Catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid |
| O43598 | 2/2 | 0.03 | 2′-deoxynucleoside 5′-phosphate N-hydrolase 1 (DNPH1) | Nucleus | 0.53 | −0.93 | Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5′-monophosphates |
| Q3L8U1 | 1/1 | 0.05 | Chromodomain-helicase-DNA-binding protein 9 (CHD9) | Nucleus | 0.52 | −0.95 | Transcriptional coactivator for PPARA and possibly other nuclear receptors |
| Q9H0R4 | 1/1 | 0.03 | Haloacid dehalogenase-like hydrolase domain-containing protein 2 (HDHD2) | ECM | 0.52 | −0.95 | Phosphotase activity |
| P06744 | 24/24 | 0.02 | Glucose-6-phosphate isomerase (GPI) | ECM | 0.52 | −0.95 | Neurotrophic factor for spinal and sensory neurons |
| P10515 | 11/11 | 0.03 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT) | Mito-chondria | 0.51 | −0.96 | Catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2 |
| P30086 | 21/20 | 0.01 | Phosphatidylethanolamine-binding protein 1 (PEBP1) | Cytoplasm | 0.51 | −0.96 | Involved in the function of the presynaptic cholinergic neurons of the central nervous system; increases the production of choline acetyltransferase but not acetylcholinesterase |
| Q9UI09 | 3/2 | 0.05 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (NDUFA12) | Mito-chondria | 0.51 | −0.96 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I) |
| Q02218 | 29/26 | 0.04 | 2-oxoglutarate dehydrogenase, mitochondrial (OGDH) | Mito-chondria | 0.51 | −0.96 | 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex |
| P09972 | 11/2 | 0.02 | Fructose-bisphosphate aldolase C (ALDOC) | Cytoplasm | 0.51 | −0.97 | Glycolysis |
| P49821 | 18/16 | 0.03 | NADH dehydrogenase [ubiquinone] flavoprotein 1 (NDUFV1) | Mito-chondria | 0.50 | −0.99 | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| O95298 | 2/2 | 0.05 | NADH dehydrogenase [ubiquinone] 1 subunit C2 (NDUFC2) | Mito-chondria | 0.50 | −0.99 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P21796 | 22/20 | 0.05 | Voltage-dependent anion-selective channel protein 1 (VDAC1) | Mito-chondria | 0.50 | −0.99 | Forms a channel through the mitochondrial outer membrane; allows diffusion of small hydrophilic molecules |
| P00918 | 15/12 | 0.05 | Carbonic anhydrase 2 (CA2) | Plasma membrane | 0.50 | −0.99 | Contributes to intracellular pH regulation |
| Q16718 | 6/6 | 0.03 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (NDUFA5) | Mito-chondria | 0.49 | −1.00 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P83111 | 6/6 | 0.04 | Serine beta-lactamase-like protein LACTB, (LACTB) | Mito-chondria | 0.49 | −1.01 | Serine protease that acts as a regulator of mitochondrial lipid metabolism |
| Q8TCA0 | 6/6 | 0.05 | Leucine-rich repeat-containing protein 20 (LRRC20) | Information not available | 0.49 | −1.01 | Information not available |
| P20674 | 8/8 | 0.04 | Cytochrome c oxidase subunit 5A, (COX5A) | Mito-chondria | 0.49 | −1.02 | This is the heme A-containing chain of cytochrome c oxidase |
| Q96A26 | 4/4 | 0.04 | Protein FAM162A (F162A) | Mito-chondria | 0.48 | −1.04 | Involved in hypoxia-induced cell death of neuronal cells |
| Q16555 | 18/13 | 0.01 | Dihydropyrimidinase-related protein 2 (DPYSL2) | Cytoplasm | 0.48 | −1.04 | Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration |
| P04179 | 9/8 | 0.00 | Superoxide dismutase [Mn], (SOD2) | Mito-chondria | 0.48 | −1.05 | Destroys superoxide anion radicals |
| O96000 | 7/7 | 0.03 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (NDUFB10) | Mito-chondria | 0.48 | −1.05 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P51649 | 12/12 | 0.03 | Succinate-semialdehyde dehydrogenase, (ALDH5A1) | Mito-chondria | 0.48 | −1.05 | Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) |
| P23458 | 1/1 | 0.05 | Tyrosine-protein kinase JAK1 (JAK1) | Cytoplasm | 0.48 | −1.06 | Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway |
| P06732 | 69/41 | 0.03 | Creatine kinase M-type (CKM) | Cytoplasm | 0.48 | −1.07 | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens |
| O95139 | 3/3 | 0.04 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (NDUFB6) | Mito-chondria | 048 | −1.07 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P40925 | 18/18 | 0.02 | Malate dehydrogenase, cytoplasmic (MDH1) | Cytoplasm | 0.47 | −1.08 | Malic enzyme activit |
| A8MU46 | 9/7 | 0.04 | Smoothelin-like protein 1 (SMTNL1) | Nucleus | 0.47 | −1.09 | Regulation of contractile properties of muscles |
| O95169 | 4/4 | 0.03 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, (NDUFB8) | Mito-chondria | 0.46 | −1.10 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P36957 | 14/12 | 0.03 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, (DLST) | Mito-chondria | 0.46 | −1.11 | Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex |
| Q9NVS9 | 2/2 | 0.01 | Pyridoxine-5′-phosphate oxidase (PNPO) | Cytoplasm | 0.46 | −1.12 | Catalyzes the oxidation of either pyridoxine 5′-phosphate (PNP) or pyridoxamine 5′-phosphate (PMP) into pyridoxal 5′-phosphate (PLP) |
| Q9NPJ3 | 3/3 | 0.00 | Acyl-coenzyme A thioesterase 13 (ACOT13) | Cytoplasm | 0.46 | −1.12 | Catalyzes the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A |
| Q9P0J0 | 7/7 | 0.05 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (NDUFA13) | Mito-chondria | 0.46 | −1.13 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P51970 | 8/8 | 0.04 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (NDUFA8) | Mito-chondria | 0.46 | −1.13 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| P00395 | 4/4 | 0.05 | Cytochrome c oxidase subunit 1 (MT-CO1) | Mito-chondria | 0.45 | −1.17 | Component of the respiratory chain that catalyzes the reduction of oxygen to water |
| P61019 | 4/4 | 0.00 | Ras-related protein Rab-2A (RAB2A) | Endoplas-mic Reticulum | 0.44 | −1.19 | Protein transport from the endoplasmic reticulum to the Golgi complex |
| O00217 | 3/3 | 0.04 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, (NDUFS8) | Mito-chondria | 0.43 | −1.20 | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| Q9UKU7 | 4/4 | 0.05 | Isobutyryl-CoA dehydrogenase, (ACAD8) | Mito-chondria | 0.43 | −1.21 | Plays a role in transcriptional coactivation within the ARC complex |
| P13798 | 8/8 | 0.00 | Acylamino-acid-releasing enzyme (APEH) | Cytoplasm | 0.43 | −1.21 | Catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid |
| P53778 | 3/2 | 0.05 | Mitogen-activated protein kinase 12 (MAPK12) | Cytoplasm | 0.42 | −1.23 | Essential component of the MAP kinase signal transduction pathway; In skeletal muscle colocalizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma |
| O43676 | 1/1 | 0.03 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (NDUFB3) | Mito-chondria | 0.41 | −1.26 | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) |
| Q9NUU6 | 1/1 | 0.02 | Inactive ubiquitin thioesterase FAM105A (FAM105A) | Information not available | 0.40 | −1.29 | Information not available |
| Q93100 | 4/3 | 0.05 | Phosphorylase b kinase regulatory subunit beta (PHKB) | Plasma membrane | 0.36 | −1.45 | Catalyzes the phosphorylation of serine in certain substrates, including troponin I |
| Q8TAT6 | 3/3 | 0.01 | Nuclear protein localization protein 4 homolog (NPLOC4) | Endoplas-mic Reticulum | 0.36 | −1.45 | Binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome |
| Q9H7C9 | 4/4 | 0.02 | Mth938 domain-containing protein (AAMDC) | Cytoplasm | 0.36 | −1.47 | Plays a role in preadipocyte differentiation and adipogenesis |
| Q8N8N7 | 5/5 | 0.02 | Prostaglandin reductase 2 (PTGR2) | Cytoplasm | 0.29 | −1.81 | Functions as 15-oxo-prostaglandin 13-reductase; overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation |
| P02792 | 4/4 | 0.01 | Ferritin light chain (FTL) | Cytoplasm | 0.27 | −1.86 | Stores iron in a soluble, non-toxic, readily available form |
| Q14558 | 1/1 | 0.01 | Phosphoribosyl pyrophosphate synthase-associated protein 1 (PRPSAP1) | Cytoplasm | 0.17 | −2.57 | Negative regulatory role in 5-phosphoribose 1-diphosphate synthesis |
| P29350 | 2/2 | 0.04 | Tyrosine-protein phosphatase non-receptor type 6 (PTPN6) | Nucleus | 0.15 | −2.73 | Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR |
| Q8NDH3 | 1/1 | 0.00 | Probable aminopeptidase NPEPL1 (NPEPL1) | Nucleus | 0.13 | −2.90 | Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides |
| P24043 | 50/48 | 0.00 | Laminin subunit alpha-2 (LAMA2) | ECM | 0.07 | −3.84 | Binding to cells via a high affinity receptor, laminin; mediates the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components |
Pep, peptides; uniq, unique. Information concerning subcellular localization and protein function were obtained from uniprot. ECM, extra cellular matrix.