Fig. 2.

Top: endogenous tau species found with N-terminal and mid-region-specific antibodies (Tau 12 in red, HT7 in blue, BT2 in orange) and neo-epitope-specific antibodies (Tau_C123 in lime, Tau_C224 in green) with selected amino acid positions indicated. Alignment and numbering refer to the 2N isoform; for peptides originating from the 0N and 1N isoforms, 2N sequence portions not included are dimmed. The protein schematic structure shows the respective antibody epitopes and T181. Bottom: in black, range (Ac-2-254) of tryptic peptides detected in CSF with Tau12 (regions with no coverage are dimmed). Vertical lines indicate possible tryptic cleavage sites