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. 2019 May 13;9:7272. doi: 10.1038/s41598-019-43573-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Synergistic effects of HUVECs and GHS interaction with leukocyte adhesion. (a) Representative images of adherent PBMNCs spread on HUVECs after 3, 6 and 24 hours were shown. PBMNCs were stained with Nucblue (red). Scale bars are 50 μm. (b) Quantification of PBMNCs adhesion rates at 3, 6 and 24 hours on Flat and GHS. ^*p < 0.05. (c) qPCR data showed expressions of MCP-1 and VCAM-1. ^*p < 0.05.