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. 2019 May 7;10:415. doi: 10.3389/fpls.2019.00415

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Copyright © 2019 Wang, Qiu, Dai, Guo, Lu, Wang, Li, Liu and Zuo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exogenous AhNRAMP1 improved Fe and Zn deficiencies in transgenic tobacco grown on calcareous soil. The phenotype (A), biomass (B), SPAD value (C), AhNRAMP1 expression profiles (D), and element concentrations (E) of tobacco in the vegetative period. NT, non-transformed plants; E-90Ω, overexpressing AhNRAMP1 transgenic plants induced by the E-90Ω promoter. The results are presented as the means ± SD of twelve replicates for SPAD value, and triplicate replicates for other data. Different lowercase letters represent significant differences of AhNRAMP1 expression level among organs at p ≤ 0.05 by Kruskal–Wallis test. Asterisks indicate significant differences with NT plants by Student’s t-Test (^∗p ≤ 0.05, ^∗∗p ≤ 0.01, and ^∗∗∗p ≤ 0.001).